Apoptosis Reagents and Kits
TRIBIOSCIENCE INC Caspase-3 Colorimetric Assay Kit, 100 Tests
Caspase-3 Colorimetric Assay provides a simple and convenient approach for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm.
Abcam Human CDK5 knockout HEK-293T cell lysate.
Human CDK5 knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human TRIP13 (PCH2) knockout HEK-293T cell lysate.
Human TRIP13 (PCH2) knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Caspase asy Buffer5X 5mL
Caspase-1 Assay Buffer (5X) has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
STEMCELL Technologies Annexin V, PE, Size: 25 Tests
Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium. This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. Verified for use with flow cytometry assays use this antibody to detect the translocation of PS to the external environment. Annexin V is used along with viability dyes such as 7-AAD (Catalog 75001) or Propidium Iodide (Catalog 75002). The process of PS translocation occurs prior to the loss of membrane integrity. Therefore as cells progress through apoptosis and towards necrosis the cell membrane is compromised and consequently viability dyes pass into the cell. Thus cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability d
Abcam Human SP3 knockout HCT116 cell lysate.
Human SP3 knockout HCT116 cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Sartorius Incucyte Caspase-3/7 Dye for Apoptosis (RED)
Caspase 3/7 Red reagent for apoptosis (4704 is RED reagent, product photo is default provided by supplier)
Cayman Chemical Z-VAD OH-FMK 500ug
An irreversible tripeptide inhibitor of all caspases; useful in studies involving recombinant, isolated, or purified enzymes
STEMCELL Technologies Annexin V Apoptosis Detection Kit with 7-AAD, APC, Size: 100 Tests
This apoptosis staining and detection kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both Annexin V and 7-AAD). It includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001). Annexin V belongs to the Annexin family of intracellular proteins that bind to membrane phospholipids in a calcium-dependent manner. This dye has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the cell membrane, exposing it to the external environment, where it is detected by Annexin V. Visit STEMCELL.com for more information and related products.
Enzo Life Sciences Z-VAD-FMK (5 mg) CAS: 220644-02-0
Pan-Caspase Inhibitor. Z-VAD-FMK acts as an effective irreversible caspase inhibitor with no cytotoxic effects and, therefore, is a useful tool for studying caspase activity. Irreversible, cell permeable broad-spectrum caspase inhibitor. Alternative Name: Caspase family inhibitor (fluoromethylketone), Z-Val-Ala-DL-Asp-FMK. Formula: C21H28FN3O7. MW: 453.5. CAS: 220644-02-0. Purity: ≥98% (HPLC). Sequence Z-Val-Ala-Asp-fluoromethylketone. Appearance: Solid. Solubility: Soluble in DMSO or acetonitrile (10mg/ml). Long Term Storage: -20°C. Use/Stability: Keep sealed after removing from the freezer until its temperature equilibrates with room temperature. Dissolve only the amount needed at one time because of the possibility of decomposition in solution. Handling: After reconstitution, prepare aliquots and store at -20°C.
Biotium NucView 488 and RedDot™ 2 Apoptosis & Necrosis Kit (100 assays)
NucView488 and RedDot™ Apoptosis/Necrosis Assay Kit contains NucView488 Caspase-3 Substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™ is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView488 Caspase-3 Substrate can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. RedDot™ is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. RedDot™ has far-red emission at 695 nm for detection in the Cy5 channel, well-separated from the green fluorescence of NucView488. The kit has reagents sufficient for 100 assays for flow cytometry.
